Fig 2: STAT2 was a target of miR-506-3p, and DLX6-AS1 regulated STAT2 expression by targeting miR-506-3p. (A) The potential target relationship between STAT2 and miR-506-3p was predicted by the online tool Starbase3.0. (B and C) The potential target relationship between STAT2 and miR-506-3p was verified by dual-luciferase reporter assay. (D and E) The expression of STAT2 in NB tissues was detected by qRT-PCR and Western blot. (F and G) The expression of STAT2 in NB cells was detected by qRT-PCR and Western blot. (H) The correlation between STAT2 expression and miR-506-3p expression in NB tissues was analyzed according to Spearman correlation analysis. (I and J) The expression of STAT2 in SK-N-SH and LAN-6 cells transfected with miR-506-3p, miR-NC, miR-506-3p+pcDNA-STAT2 or miR-506-3p+pcDNA-NC was detected by qRT-PCR and Western blot at 48 h post-transfection. (K) The correlation between STAT2 expression and DLX6-AS1 expression in NB tissues was analyzed according to Spearman correlation analysis. (L and M) The expression of STAT2 in SK-N-SH and LAN-6 cells transfected with miR-506-3p, miR-NC, miR-506-3p+pcDNA-DLX6-AS1 or miR-506-3p+pcDNA-NC was examined by qRT-PCR and Western blot at 48 h post-transfection. *P < 0.05.

Fig 3: MiR-506-3p bound to STAT2 to modulate the development of NB. SK-N-SH and LAN-6 cells were transfected with miR-506-3p, miR-NC, miR-506-3p+pcDNA-STAT2 or miR-506-3p+pcDNA-NC. (A and B) Cell proliferation was monitored at different time points using MTT assay. (C and D) Colony formation assay was performed at 48 h post-transfection. (E and F) Cell cycle was assessed by flow cytometry assay at 48 h post-transfection. (G and H) The levels of CDK1 and Cyclin D1 at 48 h post-transfection were quantified by Western blot. (I–K) Glucose production, lactate production and ATP level were checked at 48 h post-transfection to assess glycolysis using the corresponding kit. *P < 0.05.